KDM Antibodies
KDM (Lysine-specific Demethylase) Antibodies are designed to detect and bind to members of the KDM family of proteins. KDMs are a group of enzymes responsible for the removal of methyl groups from lysine residues on histones, which plays a crucial role in epigenetic regulation.
Content of KDM Antibodies
The KDM Antibodies are developed by immunizing animals (e.g., rabbits, mice) with peptides corresponding to conserved regions of the KDM proteins. KDM antibodies are available in several formats:
- Unconjugated: These are used in applications where secondary detection is applied, such as in Western blotting.
- Conjugated: Fluorescent dyes (e.g., FITC or Alexa Fluor), biotin, or enzymes like horseradish peroxidase (HRP) are commonly conjugated for direct detection in assays such as immunofluorescence or ELISA.
Application of KDM Antibodies
KDM Antibodies are widely used in epigenetic research to study the mechanisms of histone demethylation and their impact on chromatin dynamics and gene expression. Applications include:
- Chromatin Immunoprecipitation (ChIP): KDM Antibodies are frequently used in ChIP assays to investigate the recruitment of specific KDM proteins to genomic regions. This technique helps elucidate how KDMs regulate the expression of target genes by removing methyl groups from histones, which is crucial in studies of gene silencing or activation.
- Western Blotting: In Western blot analysis, KDM Antibodies detect the presence and expression levels of KDM proteins in cell lysates or tissue samples. This is particularly useful in cancer research, where aberrant expression of KDMs, such as KDM1A/LSD1 or KDM6B/JMJD3, has been implicated in tumor progression and metastasis.
- Immunofluorescence (IF) and Immunohistochemistry (IHC): KDM Antibodies can be applied to examine the localization of KDM proteins within cells or tissue sections. This is particularly valuable in studying how KDM-mediated demethylation of histones correlates with the distribution of active or repressive chromatin regions within the nucleus.
- ELISA (Enzyme-Linked Immunosorbent Assay): KDM Antibodies are used in ELISA-based assays to quantify KDM levels or investigate protein-protein interactions. ELISAs can also be used to screen for small-molecule inhibitors of KDMs, which is essential for developing targeted cancer therapies.
KDM Antibodies are indispensable tools in epigenetic research, providing insights into the role of lysine-specific demethylases in gene regulation, cellular differentiation, and disease progression.
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