ARL Antibodies

CRTC (CREB-Regulated Transcription Coactivator) antibodies target members of the CRTC family, including CRTC1, CRTC2, and CRTC3, which are transcription coactivators that modulate CREB (cAMP Response Element-Binding) protein activity. 

Content of CRTC Antibodies

CRTC antibodies are monoclonal or polyclonal antibodies that recognize specific CRTC family members. These antibodies are essential in studying the role of CRTCs in CREB-mediated gene transcription and in understanding their involvement in various cellular processes. CRTC antibodies are widely used in techniques like Western blotting, immunoprecipitation, immunofluorescence (IF), and immunohistochemistry (IHC) to detect CRTC expression and localization in tissues and cells.

• CRTC1: Predominantly expressed in the brain, plays a role in neuronal activity and synaptic plasticity.
• CRTC2: Primarily involved in glucose and lipid metabolism, regulated by insulin signaling in liver and muscle tissues.
• CRTC3: Influences metabolic pathways and energy balance, especially in adipose tissue.

Application of CRTC Antibodies

• Metabolic Research: CRTC2 and CRTC3 antibodies are crucial for studying metabolic regulation, particularly in understanding how these coactivators modulate glucose homeostasis and lipid metabolism. CRTC2 is involved in insulin signaling and hepatic glucose production, making CRTC antibodies important for research on diabetes and metabolic syndrome. CRTC3 antibodies help in exploring how this coactivator regulates adipogenesis and energy expenditure in obesity.
• Neuroscience Studies: CRTC1 is predominantly expressed in the brain and plays a significant role in neuronal plasticity, memory formation, and synaptic activity. CRTC1 antibodies are used in neuroscience research to study how CRTC1 interacts with CREB to regulate gene expression critical for learning and memory. Dysregulation of CRTC1 has been implicated in neurodegenerative diseases such as Alzheimer’s disease, making these antibodies valuable tools for exploring neurodegenerative mechanisms.
• Cancer Research: Aberrant CRTC signaling has been linked to tumor progression, especially in cancers where CREB activity is upregulated. CRTC antibodies are used to investigate how CRTC-mediated transcriptional activation contributes to cancer cell proliferation, survival, and metabolism. For example, increased CRTC2 activity has been associated with enhanced tumor growth in certain cancers.
• Immunohistochemistry and Immunofluorescence: In IHC and IF, CRTC antibodies are applied to detect the localization and expression patterns of CRTC proteins in tissues. These techniques are particularly useful for studying how CRTCs respond to physiological and pathological stimuli, such as stress signals or metabolic changes, in different tissues, including the liver, brain, and adipose tissue.

CRTC antibodies are indispensable tools for studying transcriptional regulation, metabolic control, neuronal function, and cancer progression. These antibodies contribute to the understanding of CREB-mediated gene transcription and its impact on cellular responses in both normal and disease states.