CACNG Antibodies
CACNG antibodies target the gamma subunit of voltage-dependent calcium channels, specifically from the CACNG family, which includes eight known subunits (CACNG1 through CACNG8). These subunits play a critical role in modulating the activity and biophysical properties of calcium channels, particularly in the context of excitatory synaptic transmission. CACNG proteins are involved in the stabilization and regulation of the calcium channel complex, influencing channel gating, trafficking, and voltage sensitivity. Dysregulation of CACNG proteins is linked to various neurological disorders, including epilepsy and myasthenia gravis.
Application of CACNG Antibodies
- Immunofluorescence (IF): CACNG antibodies are employed in IF studies to visualize the subcellular localization of gamma subunits in neurons or muscle cells. This is essential for understanding how CACNG subunits influence calcium channel trafficking and function at synapses. IF with CACNG antibodies provides insights into the cellular and molecular mechanisms regulating calcium homeostasis in excitatory synapses.
- Flow Cytometry (FACS): Fluorophore-conjugated CACNG antibodies are used in flow cytometry to quantify the expression of CACNG subunits in different cell populations. This application is valuable for studying calcium channel regulation in various tissues, including neurons, cardiomyocytes, and skeletal muscle cells. Flow cytometry with CACNG antibodies is particularly useful in high-throughput analyses of genetic models with altered calcium channel function.
- Co-Immunoprecipitation (Co-IP): CACNG antibodies are used in Co-IP assays to investigate protein-protein interactions involving CACNG subunits and other calcium channel components. This application helps elucidate how CACNG subunits interact with other regulatory proteins and influence calcium channel assembly and stability. Co-IP studies with CACNG antibodies are essential for understanding the molecular dynamics of voltage-gated calcium channels.
- Enzyme-Linked Immunosorbent Assay (ELISA): ELISA-based applications using CACNG antibodies allow for the quantification of CACNG subunit expression levels in cell lysates or biological fluids. This method is useful for assessing changes in CACNG protein levels under various conditions, including disease models where calcium channel dysfunction is a contributing factor.
Technical Specifications
- Clonality: Monoclonal or Polyclonal
- Isotype: IgG (species-specific variants)
- Species Reactivity: Human, Mouse, Rat, and others depending on the specific antibody
- Applications: WB, IHC, IF, FACS, Co-IP, ELISA
- Isoform Specificity: Available for specific CACNG subunits (e.g., CACNG1, CACNG2)
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