CA8 Antibodies

CA8 (Carbonic Anhydrase-Related Protein 8) antibodies target the CA8 protein, which is part of the carbonic anhydrase family but lacks catalytic activity typically associated with other members of this family. Unlike other carbonic anhydrases, CA8 functions in the regulation of calcium signaling, particularly within the cerebellum. Mutations in the CA8 gene are linked to neurological disorders such as cerebellar ataxia. CA8 plays a critical role in Purkinje cell function, and its dysregulation affects motor coordination and other cerebellar processes.

Application of CA8 Antibodies

1. Immunofluorescence (IF): CA8 antibodies are commonly employed in immunofluorescence to visualize the subcellular localization of CA8, particularly in neurons. This application is critical for studying the role of CA8 in calcium signaling and how its dysregulation affects neuronal function. IF with CA8 antibodies allows researchers to track CA8 expression in various cellular compartments and examine how mutations in CA8 impact its localization and function in neurological diseases.
2. Flow Cytometry (FACS): Fluorophore-conjugated CA8 antibodies are used in flow cytometry to quantify the expression of CA8 in different cell populations. This application is useful for high-throughput analysis of CA8 protein levels, particularly in research related to cerebellar function or developmental studies involving Purkinje cells.
3. Co-Immunoprecipitation (Co-IP): CA8 antibodies are used in Co-IP assays to investigate the protein-protein interactions involving CA8. This helps in understanding how CA8 interacts with other calcium signaling proteins or molecular partners in the cerebellum. Co-IP with CA8 antibodies is particularly useful for elucidating the signaling pathways and molecular complexes CA8 is involved in, especially in the context of neurodegenerative conditions.
4. Enzyme-Linked Immunosorbent Assay (ELISA): CA8 antibodies are also used in ELISA to quantify CA8 protein levels in biological fluids or cell lysates. ELISA-based assays are important for evaluating CA8 expression in disease models and for assessing the effects of genetic mutations on CA8 protein levels in patients with cerebellar ataxia.

Technical Specifications

• Clonality: Monoclonal or Polyclonal
• Isotype: IgG (species-specific variants)
• Species Reactivity: Human, Mouse, Rat, with cross-reactivity in other species depending on the antibody
• Applications: WB, IHC, IF, FACS, Co-IP, ELISA
• Storage Conditions: Typically stored at -20°C or -80°C for long-term stability