C18ORF Antibodies

C18ORF antibodies target proteins encoded by genes located on chromosome 18 open reading frames (C18ORF), which belong to a family of genes involved in various cellular processes, such as autophagy, intracellular trafficking, and mitochondrial function. C18ORF proteins are typically identified by their gene locus designation, and they are often involved in critical biological pathways, including lysosomal degradation and endosomal dynamics. One of the most studied members of this family is C18ORF8, which is linked to immunity and inflammation via the C18ORF8-RAB7-GTPase pathway.

Application of C18ORF Antibodies

• Immunofluorescence (IF): C18ORF antibodies are also utilized in IF to examine the subcellular localization of C18ORF proteins. This is particularly valuable for understanding their role in autophagy, endosomal trafficking, or lysosomal function. Immunofluorescence can reveal how C18ORF proteins interact with other cellular compartments, such as mitochondria or endosomes, providing insights into their function in intracellular dynamics.
• Flow Cytometry (FACS): Flow cytometry, using fluorophore-conjugated C18ORF antibodies, allows for the quantification of C18ORF protein expression in cell populations. This application is useful in studying the role of C18ORF proteins in immune cells, particularly in relation to their involvement in inflammatory responses and autophagy. FACS with C18ORF antibodies can identify shifts in protein expression that are important in disease states like cancer or inflammation.
• Co-Immunoprecipitation (Co-IP): C18ORF antibodies are often employed in Co-IP assays to investigate the interactions between C18ORF proteins and other intracellular proteins. This application helps elucidate the signaling pathways regulated by C18ORF proteins, especially in processes related to autophagy, lysosomal function, or mitochondrial regulation.
• Enzyme-Linked Immunosorbent Assay (ELISA): C18ORF antibodies are applied in ELISA to quantify C18ORF protein levels in cell lysates or biological fluids. This high-throughput method allows for the assessment of C18ORF expression under various conditions, making it valuable for diagnostic and therapeutic research, particularly in diseases where C18ORF protein dysregulation plays a role.

Technical Specifications

• Clonality: Monoclonal or Polyclonal
• Isotype: IgG (species-specific variants)
• Species Reactivity: Human, Mouse, Rat, with potential cross-reactivity to other species
• Applications: WB, IHC, IF, FACS, Co-IP, ELISA
• Epitope Specificity: Available for detecting specific C18ORF proteins (e.g., C18ORF8)
• Storage Conditions: Typically stored at -20°C or -80°C for long-term stability