HOXA Antibodies

ARL Antibodies

HOXA antibodies are specific to the proteins encoded by the HOXA cluster, a subgroup within the Homeobox (HOX) gene family. The HOXA cluster consists of 11 genes (HOXA1 to HOXA13) located on chromosome 7 in humans, which play pivotal roles in the regulation of embryonic development and cell differentiation. HOXA genes are involved in specifying positional identity along the anterior-posterior axis, guiding proper tissue patterning during organogenesis.

Content of HOXA Antibodies

HOXA antibodies are developed to specifically bind to various HOXA proteins, recognizing unique or conserved sequences within each isoform. 

Key specifications:

• Immunogen: Synthetic peptides or recombinant proteins corresponding to specific regions of HOXA proteins.
• Host: Typically raised in rabbits or mice.
• Applications: Suitable for Western blotting (WB), immunohistochemistry (IHC), immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), and flow cytometry (FC).
• Reactivity: HOXA antibodies usually react with human, mouse, and rat proteins, but may exhibit cross-reactivity in other species depending on the isoform.

Application of HOXA Antibodies

HOXA antibodies are essential for research in developmental biology, cancer biology, and stem cell research. Their applications include:

• Western Blotting (WB): HOXA antibodies are used to detect specific HOXA proteins in cell lysates or tissue extracts, helping to identify changes in HOXA protein expression under different physiological or pathological conditions, including during embryonic development and in various cancers.
• Immunohistochemistry (IHC): By using HOXA antibodies, researchers can visualize the spatial distribution of HOXA proteins in tissue sections. 
• Chromatin Immunoprecipitation (ChIP): HOXA antibodies are employed in ChIP assays to study HOXA protein binding to DNA, helping to elucidate their role in gene regulation. .
• Flow Cytometry (FC): HOXA antibodies can be conjugated to fluorescent labels for use in flow cytometry, allowing the quantification and analysis of HOXA protein expression in different cell populations, including stem cells and cancer cells.

The study of HOXA antibodies contributes to understanding their roles in development, stem cell maintenance, and their dysregulation in cancer, making these antibodies crucial for developmental and clinical research.