ADRB2 Antibodies

ADRB2 antibodies are designed to target the Beta-2 Adrenergic Receptor (ADRB2), a G-protein-coupled receptor (GPCR) that binds adrenaline and noradrenaline. ADRB2 is primarily involved in the regulation of smooth muscle relaxation, bronchial dilation, cardiac function, and metabolic processes. 

Content

ADRB2 plays a critical role in the sympathetic nervous system's response to stress, mediating effects like increased heart rate, relaxation of airway smooth muscles, and energy mobilization. Upon activation by catecholamines, ADRB2 stimulates the Gs protein, leading to the activation of adenylate cyclase and an increase in cyclic AMP (cAMP) levels, which triggers downstream signaling cascades. 

Application

ADRB2 antibodies are applied in various research and diagnostic techniques, including:

• Immunohistochemistry (IHC): Employed to localize ADRB2 within tissue sections, providing insights into its distribution across different tissues, particularly in the lungs, heart, and skeletal muscles. IHC is useful for examining how receptor expression varies under normal and pathological conditions, such as asthma and heart failure.
• Immunofluorescence (IF): Enables detailed visualization of ADRB2 at the cellular level, allowing researchers to study receptor localization, trafficking, and interactions with other cellular components. IF is particularly helpful for examining receptor internalization and recycling dynamics after activation.
• Flow Cytometry: Used to analyze ADRB2 expression on the surface of various cell types, facilitating studies on receptor density and response to agonists or antagonists. Flow cytometry with ADRB2 antibodies can be useful in assessing receptor function in immune cells, smooth muscle cells, and cardiac cells.
• Immunoprecipitation (IP): Applied to isolate ADRB2 from complex protein mixtures, enabling the study of receptor interactions with signaling molecules, adaptor proteins, and other GPCRs. IP can be used to investigate how ADRB2 signaling is modulated under different conditions and in response to therapeutic agents.

These applications make ADRB2 antibodies vital tools for advancing research on the physiological and pathological roles of the Beta-2 Adrenergic Receptor. They contribute to understanding the receptor's involvement in cardiovascular health, respiratory function, and metabolic regulation, supporting the development of therapeutic strategies for conditions like asthma, hypertension, and metabolic disorders.