ADORA1 Antibodies

ADORA1 antibodies are developed to specifically target the Adenosine A1 Receptor (ADORA1), a G-protein-coupled receptor (GPCR) that binds adenosine, a neuromodulator involved in various physiological processes. ADORA1 plays a key role in regulating neurotransmitter release, heart rate, and renal function. It is primarily expressed in the brain, heart, and kidneys, where it mediates the inhibitory effects of adenosine. 

Content

ADORA1 is one of four subtypes of adenosine receptors (A1, A2A, A2B, A3) and is primarily associated with inhibitory signaling. It couples with Gi/o proteins to inhibit adenylate cyclase activity, leading to reduced cAMP production. This inhibitory signaling mechanism helps regulate neurotransmitter release in the central nervous system, modulate heart rate, and control renal blood flow and function. 

Application

ADORA1 antibodies are widely used in multiple research applications, such as:

• Immunohistochemistry (IHC): Employed to localize ADORA1 in tissue sections, providing insights into its distribution within the brain, heart, and kidneys. IHC with ADORA1 antibodies helps in understanding how receptor expression changes in different diseases, such as Parkinson’s disease, epilepsy, and heart failure.
• Immunofluorescence (IF): Allows for the detailed observation of ADORA1 at the cellular level, offering insights into its localization and interaction with other proteins. IF is particularly useful in studying receptor trafficking, localization in synaptic regions, and receptor modulation during cellular stress.
• Flow Cytometry: Used to analyze ADORA1 expression on the surface of cells, facilitating research into cell-specific receptor distribution and studying how ADORA1 expression changes in response to various stimuli or treatments.
• Immunoprecipitation (IP): Applied to isolate ADORA1 from complex protein mixtures, enabling the study of receptor-protein interactions and signaling mechanisms. IP can be used to explore how different ligands or drugs influence receptor binding and downstream signaling pathways.

These applications make ADORA1 antibodies valuable tools for advancing research in neurobiology, cardiology, and nephrology. By enabling the detailed study of ADORA1 expression and function, they contribute to understanding the mechanisms of diseases like neurodegeneration, cardiovascular disorders, and hypertension, supporting the development of targeted therapies that modulate adenosine signaling.