ADAR Antibodies

ADAR (adenosine deaminase acting on RNA) antibodies are used in research to detect and study the ADAR family of enzymes, which are involved in RNA editing processes. RNA editing by ADARs converts adenosine residues into inosine in double-stranded RNA (dsRNA), affecting the expression and function of various genes. This process is essential for the regulation of gene expression, RNA stability, and the innate immune response.

Types and Functions of ADAR Enzymes

• ADAR1:
  • Isoforms: Two major isoforms, ADAR1 p110 (constitutively expressed) and ADAR1 p150 (inducible by interferons).
  • Functions: Involved in editing of viral and cellular dsRNA, regulation of immune response, and maintenance of cellular homeostasis.
• ADAR2:
  • Expression: Primarily in the nervous system.
  • Functions: Edits neurotransmitter receptor transcripts, affecting neural function and plasticity.
• ADAR3:
  • Expression: Brain-specific, functions less understood compared to ADAR1 and ADAR2.

Applications of ADAR Antibodies

• Western Blotting:
  • Purpose: Detect and quantify ADAR protein levels in cell or tissue extracts.
  • Application: Study expression patterns, isoform-specific expression, and responses to various stimuli.
• Immunohistochemistry (IHC):
  • Purpose: Localize ADAR proteins in tissue sections.
  • Application: Investigate the tissue-specific distribution of ADARs and their subcellular localization.
• Immunoprecipitation (IP):
  • Purpose: Enrich ADAR proteins from cell lysates.
  • Application: Study protein-protein interactions and post-translational modifications.
• Enzyme-Linked Immunosorbent Assay (ELISA):
  • Purpose: Quantify ADAR protein levels in biological samples.
  • Application: Measure changes in ADAR expression under different conditions

​