ABCD1 Antibodies

ABCD1 antibodies are developed to specifically target the ATP-Binding Cassette Subfamily D Member 1 (ABCD1) protein, which is a transporter located in the peroxisomal membrane. ABCD1 is essential for the transport of very-long-chain fatty acids (VLCFAs) into peroxisomes, where they undergo beta-oxidation. Mutations in the ABCD1 gene cause X-linked adrenoleukodystrophy (X-ALD), a genetic disorder characterized by the accumulation of VLCFAs, leading to severe neurological and adrenal dysfunction. 

Content

ABCD1 is a peroxisomal membrane protein that plays a key role in lipid metabolism. It functions as a transporter that facilitates the import of VLCFAs into peroxisomes for degradation through beta-oxidation. Proper functioning of ABCD1 is essential for maintaining lipid homeostasis and preventing the toxic accumulation of VLCFAs. Mutations in the ABCD1 gene lead to impaired transport activity, resulting in the buildup of VLCFAs in cells, particularly affecting the nervous system and adrenal glands. 

Application

ABCD1 antibodies are utilized in various research applications, including:

• Immunofluorescence (IF): Allows for high-resolution visualization of ABCD1 at the cellular level, providing insights into its precise localization within peroxisomes. IF is useful for studying the dynamics of ABCD1 and its interaction with other peroxisomal proteins, as well as changes in response to different treatments or stress conditions.
• Flow Cytometry: Applied to analyze ABCD1 expression on a per-cell basis, helping quantify changes in protein levels under various experimental conditions, such as drug treatments aimed at modulating ABCD1 function.
• Immunoprecipitation (IP): Used to isolate ABCD1 from complex protein mixtures, facilitating the study of its interactions with other proteins involved in peroxisomal lipid metabolism. IP can help identify regulatory mechanisms that affect ABCD1 function and its role in maintaining cellular lipid balance.

These applications make ABCD1 antibodies essential tools for advancing research on peroxisomal function, lipid metabolism, and related genetic disorders. They enable scientists to investigate how ABCD1 mutations lead to the accumulation of VLCFAs and contribute to the development of X-ALD.