AffiAB® Mouse Anti-Human IgG (class specific)
Host
Mouse.
Isotype
NI 335 and NI 343.
Isotypet
IgG1.
Formulation
Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2) . No preservative added, as it may interfere with the Antibody activity. No foreign protein added. IgG concentration is 0.4 mg/ml. No foreign proteins added.
Shipping Conditions
N/A.
Storage Conditions
To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG, and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.