AffiAB®Anti-GFP VHH Antibody, biotinylated

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CAT# AFG-JTB-001
Size: 100 µg
Conjugation: Conjugated

375.00 375.0 USD 375.00

375.00

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AffiAB®Anti-GFP VHH Antibody, biotinylated

Specificity 

Green fluorescent protein (GFP)

Species Reactivity

Species independent

Description

Alpaca derived anti-Ubiquitin VHH single domain antibody (molecular weight: 14.0 kDa), expressed in E. coli under conditions free from animal derived components


Clone

GE10

Conjugate and tags 

A 6*His tag at its C-terminal, followed by an Avi tag. The single lysine residue in the Avi tag is enzymatically labeled with one biotin.

Tested Applications

ELISA, WB

Purification

Affinity chromatography purified via Ni-charged resin

Format

Liquid

Buffer solution

25 mM TAPS pH8.5, 500 mM NaCl, 5 mM EDTA, 0.1 % Proclin 300 Solution


Recommended dilutions

​ELISA 1:9000-1:200000

WB 1:500-1:5000

4°C; Do not freeze.

Green fluorescent protein (GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light (emission peak at 509nm) when excited by blue light (excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr (previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488 nm.