AffiAB® 647 Conjugated Goat anti-rabbit IgG Goat Polyclonal Antibody (HA1123)

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CAT# AFG-HB-4300
Size: 100 μL

305.10 305.1 USD 305.10

305.10

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AffiAB® 647 Conjugated Goat anti-rabbit IgG Goat Polyclonal Antibody (HA1123)

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. iFluor™ 594 dyes have fluorescence excitation and emission maxima of ~590 nm and ~610 nm respectively. iFluor™ 594 family is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family an excellent alternative. iFluor™ 594 is much easier to be conjugated with RPE with much higher conjugation yield, and the resulted RPE-iFluor™ 594 tandem has better FRET efficiency. iFluor™ 594 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups.

Antibody type

Goat polyclonal secondary

Uniprot ID

N/A

Recombinant

FALSE

Conjugation

iFluor™ 647

Host

Goat

Isotype

IgG

Clone

N/A

KO/KD

N/A

Species reactivity

Rabbit

Tested applications

IF-Cell, IF-Tissue

Predicted species reactivity

N/A

Immunogen

Rabbit IgG (H+L) .

Storage

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Form

Liquid

Storage buffer

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS

Concentration

1ug/ul

Purity

Immunogen affinity purified.

Signal pathway

N/A

Recommended dilutions

IF-Cell: 1:500-1:1, 000; IF-Tissue: 1:500-1:1, 000

Molecular Weight

N/A

Subcellular location

N/A

Positive control

N/A