AffiAB® 647 Conjugated Anti-Sodium Potassium ATPase Antibody [ST0533]

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CAT# AFG-HB-4297
Size: 100 μL

386.10 386.1 USD 386.10

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AffiAB® 647 Conjugated Anti-Sodium Potassium ATPase Antibody [ST0533]

The sodium–potassium pump (sodium–potassium adenosine triphosphatase, also known as Na⁺/K⁺-ATPase, Na⁺/K⁺ pump, or sodium–potassium ATPase) is an enzyme (an electrogenic transmembrane ATPase) found in the membrane of all animal cells. It performs several functions in cell physiology. The Na⁺/K⁺-ATPase helps maintain resting potential, affects transport, and regulates cellular volume. It also functions as a signal transducer/integrator to regulate the MAPK pathway, reactive oxygen species (ROS) , as well as intracellular calcium. In fact, all cells expend a large fraction of the ATP they produce (typically 30% and up to 70% in nerve cells) to maintain their required cytosolic Na and K concentrations. For neurons, the Na⁺/K⁺-ATPase can be responsible for up to 3/4 of the cell's energy expenditure. In many types of tissue, ATP consumption by the Na⁺/K⁺-ATPases have been related to glycolysis. This was first discovered in red blood cells (Schrier, 1966) , but has later been evidenced in renal cells, smooth muscles surrounding the blood vessels, [6] and cardiac purkinje cells. Recently, glycolysis has also been shown to be of particular importance for Na⁺/K⁺-ATPases in skeletal muscles, where inhibition of glycogen breakdown (a substrate for glycolysis) leads to reduced Na⁺/K⁺-ATPase activity and lower force production.

Antibody type

Recombinant Rabbit monoclonal Antibody

Uniprot ID

SwissProt: P05023 Human; SwissProt: P05026 Human; SwissProt: Q13733 Human; SwissProt: P06685 Rat; SwissProt: P07340 Rat; SwissProt: Q64541 Rat

Recombinant

YES

Conjugation

iFluor™ 647

Host

Rabbit

Isotype

IgG

Clone

ST0533

KO/KD

N/A

Species reactivity

Human, Mouse, Rat

Tested applications

IF-Tissue, FC

Predicted species reactivity

N/A

Immunogen

Synthetic peptide within Human ATP1A1 aa 39-83 / 1023.

Storage

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Form

Liquid

Storage buffer

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.

Concentration

1 mg/mL.

Purity

Protein A affinity purified.

Signal pathway

N/A

Recommended dilutions

IF-Tissue: 1:100; FC: 1:500-1:1, 000

Molecular Weight

100 kDa

Subcellular location

Cell membrane, Melanosome.

Positive control

Rat kidney tissue, human liver tissue, Hela.