AffiAB® 647 Conjugated Anti-Lamin B1 Antibody [SI17-07]

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CAT# AFG-HB-4294
Size: 100 μL

386.10 386.1 USD 386.10

386.10

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AffiAB® 647 Conjugated Anti-Lamin B1 Antibody [SI17-07]

A unique family of cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, termed Ced-3/ICE, functions as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Nuclear lamins are critical to maintaining the integrity of the nuclear envelope and cellular morphology as components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. Nuclear Lamin B is fragmented as a consequence of apoptosis by an unidentified member of the ICE family.

Antibody type

Recombinant Rabbit monoclonal Antibody

Uniprot ID

SwissProt: P20700 Human; SwissProt: P70615 Rat

Recombinant

YES

Conjugation

iFluor™ 647

Host

Rabbit

Isotype

IgG

Clone

SI17-07

KO/KD

N/A

Species reactivity

Human, Mouse, Rat

Tested applications

IF-Tissue

Predicted species reactivity

N/A

Immunogen

Synthetic peptide within Human Lamin B1 aa 511-560 / 586.

Storage

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Form

Liquid

Storage buffer

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS

Concentration

1 mg/mL.

Purity

Protein A affinity purified.

Signal pathway

N/A

Recommended dilutions

IF-Tissue: 1:200

Molecular Weight

Predicted band size: 66 kDa

Subcellular location

Nucleus inner membrane.

Positive control

Rat large intestine tissue, human colon carcinoma tissue, human breast carcinoma tissue.