AffiAB® 647 Conjugated Anti-Cytokeratin 4 Antibody [SN74-03]
Cytokeratins are a subfamily of intermediate filament keratins that are characterized by a remarkable biochemical diversity, which is represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in isoelectric range between 4.9 and 7.8. Cytokeratin 1 has the highest molecular weight, while Cytokeratin 19 has the lowest molecular weight. The cytokeratins are divided into the type I and type II subgroups. Type II family members comprise the basic to neutral members, Cytokeratins 1-8, while the type I group comprises the acidic members, Cytokeratins 9-20. Various epithelia in the human body usually express cytokeratins which are characteristic of the type of epithelium and related to the degree of maturation or differentiation within said epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. Cytokeratin 4 is expressed in differentiated layers of the mucosal and esophageal epithelia along with Cytokeratin 13.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P19013 Human
Recombinant
YES
Conjugation
iFluor™ 647
Host
Rabbit
Isotype
IgG
Clone
SN74-03
KO/KD
N/A
Species reactivity
Human
Tested applications
IF-Cell, IF-Tissue
Predicted species reactivity
N/A
Immunogen
Recombinant protein within Human Cytokeratin 4 aa 395-520.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
IF-Cell: 1:100; IF-Tissue: 1:200
Molecular Weight
Predicted band size: 56 kDa
Subcellular location
Cell surface, intermediate filament cytoskeleton, nucleus.
Positive control
A431, human tonsil tissue.