AffiAB® 594 Conjugated Goat anti-rabbit IgG Goat Polyclonal Antibody (HA1122)
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. iFluor™ 594 dyes have fluorescence excitation and emission maxima of ~590 nm and ~610 nm respectively. iFluor™ 594 family is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family an excellent alternative. iFluor™ 594 is much easier to be conjugated with RPE with much higher conjugation yield, and the resulted RPE-iFluor™ 594 tandem has better FRET efficiency. iFluor™ 594 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups.
Antibody type
Goat polyclonal secondary
Uniprot ID
N/A
Recombinant
FALSE
Conjugation
iFluor™ 594
Host
Goat
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Rabbit
Tested applications
IF-Cell, IF-Tissue, FC
Predicted species reactivity
N/A
Immunogen
Rabbit IgG (H+L) .
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
Concentration
1ug/ul
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
IF-Cell: 1:500-1:1, 000; IF-Tissue: 1:500-1:1, 000; FC: 1:500-1:1, 000
Molecular Weight
N/A
Subcellular location
N/A
Positive control
N/A