AffiAB® 488 Conjugated Goat anti-rabbit IgG Goat Polyclonal Antibody (HA1121)

https://www.affiab.com/web/image/product.template/116635/image_1920?unique=d674372

CAT# AFG-HB-4258
Size: 100 μL

305.10 305.1 USD 305.10

305.10

For Price Contact info@affigen.com

This combination does not exist.

Terms and Conditions
30-day money-back guarantee
Shipping: 2-3 Business Days

AffiAB® 488 Conjugated Goat anti-rabbit IgG Goat Polyclonal Antibody (HA1121)

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Although FITC is still the most popular fluorescent labeling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence. Protein conjugates prepared with iFluor™ 488 dyes are far superior compared to conjugates of fluorescein derivatives such as FITC. iFluor™ 488 conjugates are significantly brighter than fluorescein conjugates and are much more photostable. Additionally, the fluorescence of iFluor™ 488 is not affected by pH (4-10) . This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only at pH above 9. iFluor™ 488 SE dye is reasonably stable and shows good reactivity and selectivity with protein amino groups.

Antibody type

Goat polyclonal secondary

Uniprot ID

N/A

Recombinant

FALSE

Conjugation

iFluor™ 488

Host

Goat

Isotype

IgG

Clone

N/A

KO/KD

N/A

Species reactivity

Rabbit

Tested applications

IF-Cell, IF-Tissue, FC

Predicted species reactivity

N/A

Immunogen

Rabbit IgG (H+L) .

Storage

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Form

Liquid

Storage buffer

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS

Concentration

1ug/ul

Purity

Immunogen affinity purified.

Signal pathway

N/A

Recommended dilutions

IF-Cell: 1:500-1:1, 000; IF-Tissue: 1:500-1:1, 000; FC: 1:500-1:1, 000

Molecular Weight

N/A

Subcellular location

N/A

Positive control

N/A