AffiAB® 488 Conjugated Goat anti-rabbit IgG Goat Polyclonal Antibody (HA1121)

https://www.affiab.com/web/image/product.template/116635/image_1920?unique=23aa515

CAT# AFG-HB-4258
Size: 100 μL

305.10 305.1 USD 305.10

305.10

For Price contact us on info@gentaur.com

    This combination does not exist.

    Terms and Conditions
    14-day money-back guarantee
    Shipping: 1-3 Weeks

    AffiAB® 488 Conjugated Goat anti-rabbit IgG Goat Polyclonal Antibody (HA1121)

    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Although FITC is still the most popular fluorescent labeling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence. Protein conjugates prepared with iFluor™ 488 dyes are far superior compared to conjugates of fluorescein derivatives such as FITC. iFluor™ 488 conjugates are significantly brighter than fluorescein conjugates and are much more photostable. Additionally, the fluorescence of iFluor™ 488 is not affected by pH (4-10) . This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only at pH above 9. iFluor™ 488 SE dye is reasonably stable and shows good reactivity and selectivity with protein amino groups.

    Antibody type

    Goat polyclonal secondary

    Uniprot ID

    N/A

    Recombinant

    FALSE

    Conjugation

    iFluor™ 488

    Host

    Goat

    Isotype

    IgG

    Clone

    N/A

    KO/KD

    N/A

    Species reactivity

    Rabbit

    Tested applications

    IF-Cell, IF-Tissue, FC

    Predicted species reactivity

    N/A

    Immunogen

    Rabbit IgG (H+L) .

    Storage

    Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

    Form

    Liquid

    Storage buffer

    Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS

    Concentration

    1ug/ul

    Purity

    Immunogen affinity purified.

    Signal pathway

    N/A

    Recommended dilutions

    IF-Cell: 1:500-1:1, 000; IF-Tissue: 1:500-1:1, 000; FC: 1:500-1:1, 000

    Molecular Weight

    N/A

    Subcellular location

    N/A

    Positive control

    N/A

    Related Products

    Your Dynamic Snippet will be displayed here... This message is displayed because you did not provided both a filter and a template to use.