AffiAB® 488 Conjugated Anti-Vimentin Antibody [SC60-05]

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CAT# AFG-HB-4256
Size: 100 μL

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    AffiAB® 488 Conjugated Anti-Vimentin Antibody [SC60-05]

    Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types: fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive. Mesoderm derived carcinomas like renal cell carcinoma, adrenal cortical carcinoma and adenocarcinomas from endometrium and ovary usually express vimentin. Also thyroid carcinomas are vimentin positive. Any low differentiated or sarcomatoid carcinoma may express some vimentin. Vimentin is frequently included in the so-called primary panel (together with CD45, cytokeratin, and S-100 protein) : Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is strongly suggestive of a mesenchymal tumour or a malignant melanoma. However, in biopsies representing only a sarcomatoid part of renal cell carcinoma a.o. a strong positivity for vimentin without cytokeratin expression may be seen. Tumours like lymphomas and seminomas have the same intermediate filament profile, but the vimentin expression is usually weaker.

    Antibody type

    Recombinant Rabbit monoclonal Antibody

    Uniprot ID

    SwissProt: P08670 Human; SwissProt: P20152 Mouse; SwissProt: P31000 Rat

    Recombinant

    YES

    Conjugation

    iFluor™ 488

    Host

    Rabbit

    Isotype

    IgG

    Clone

    SC60-05

    KO/KD

    N/A

    Species reactivity

    Human, Mouse, Rat

    Tested applications

    IF-Cell, IF-Tissue, FC

    Predicted species reactivity

    N/A

    Immunogen

    Synthetic peptide within C-terminal human Vimentin.

    Storage

    Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Form

    Liquid

    Storage buffer

    Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.

    Concentration

    1 mg/mL.

    Purity

    Protein A affinity purified.

    Signal pathway

    N/A

    Recommended dilutions

    IF-Cell: 1:100; IF-Tissue: 1:200; FC: 1:1, 000

    Molecular Weight

    Predicted band size: 54 kDa

    Subcellular location

    Cytoplasm.

    Positive control

    C2C12, Hela, L6, human kidney tissue, human pancreas tissue, human appendix tissue.

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