AffiAB® 488 Conjugated Anti-Collagen VI Antibody [SD83-03]

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CAT# AFG-HB-4230
Size: 100 μL

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386.10

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    AffiAB® 488 Conjugated Anti-Collagen VI Antibody [SD83-03]

    This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.

    Antibody type

    Recombinant Rabbit monoclonal Antibody

    Uniprot ID

    SwissProt: P12109 Human; SwissProt: P12110 Human; SwissProt: P12111 Human; SwissProt: Q02788 Mouse; SwissProt: Q04857 Mouse

    Recombinant

    YES

    Conjugation

    iFluor™ 488

    Host

    Rabbit

    Isotype

    IgG

    Clone

    SD83-03

    KO/KD

    N/A

    Species reactivity

    Human, Mouse, Rat

    Tested applications

    IF-Cell, IF-Tissue

    Predicted species reactivity

    N/A

    Immunogen

    Recombinant protein within Human Collagen VI aa 17-255 / 1, 028.

    Storage

    Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

    Form

    Liquid

    Storage buffer

    Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS

    Concentration

    1 mg/mL.

    Purity

    Protein A affinity purified.

    Signal pathway

    N/A

    Recommended dilutions

    IF-Cell: 1:100; IF-Tissue: 1:50

    Molecular Weight

    Predicted band size: 109 kDa

    Subcellular location

    Extracellular matrix.

    Positive control

    Hela, mouse colon tissue.

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