AffiAB® 488 Conjugated Anti-Calretinin Antibody [JM12-93]

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CAT# AFG-HB-4227
Size: 100 μL

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AffiAB® 488 Conjugated Anti-Calretinin Antibody [JM12-93]

Calbindin D28K and Calretinin (also designated CR or 29 kDa Calbindin) are two closely related intracellular calcium-binding proteins belonging to the Troponin-C superfamily. Initially isolated from chick retina, Calretinin shares 58% identical residues with human Calbindin D28K. Calretinin is expressed in the brain and is particularly abundant in auditory neurons with precisely timed discharges. Neurons in the nucleus accumbens containing Calretinin all possess nuclear indentations. Calretinin-immunoreactive boutons form asymmetrical and symmetrical synaptic specializations on spines, dendrites and somata. The symmetrical synaptic specializations have medium-sized spiny neurons and contact other Calretinin-immunoreactive somata. Calretinin is widely used as a immunocytochemical marker for mesothelioma.

Antibody type

Recombinant Rabbit monoclonal Antibody

Uniprot ID

SwissProt: P22676 Human

Recombinant

YES

Conjugation

iFluor™ 488

Host

Rabbit

Isotype

IgG

Clone

JM12-93

KO/KD

N/A

Species reactivity

Human

Tested applications

IF-Cell, FC

Predicted species reactivity

N/A

Immunogen

Synthetic peptide within human Calretinin aa 60-100.

Storage

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Form

Liquid

Storage buffer

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS

Concentration

1 mg/mL.

Purity

Protein A affinity purified.

Signal pathway

N/A

Recommended dilutions

IF-Cell: 1:100; FC: 1:500-1:1, 000

Molecular Weight

Predicted band size: 32 kDa

Subcellular location

Cuticular plate, cytosol, nucleus, synaptic membrane, dendrite, gap junction, neuron projection, parallel fiber to Purkinje cell synapse, stereocilium, synapse, terminal bouton.

Positive control

SH-SY5Y.