AffiAB® 488 Conjugated Anti-Beta III Tubulin Antibody [A8-D10]

https://www.affiab.com/web/image/product.template/116602/image_1920?unique=136d80a

CAT# AFG-HB-4225
Size: 100 μL

354.60 354.6 USD 354.60

354.60

For Price contact us on info@gentaur.com

    This combination does not exist.

    Terms and Conditions
    14-day money-back guarantee
    Shipping: 1-3 Weeks

    AffiAB® 488 Conjugated Anti-Beta III Tubulin Antibody [A8-D10]

    Class III β-tubulin, otherwise known as βIII-tubulin (β3-tubulin) or β-tubulin III, is a microtubule element of the tubulin family. In humans, it is encoded by the TUBB3 gene. It is possible to use monoclonal antibodies and immunohistochemistry to identify neurons in samples of brain tissue, separating neurons from glial cells, which do not express Class III β-tubulin. Class III β-tubulin is one of the seven β-tubulin isotypes identified in the human genome, predominantly in neurons and the testis. It is conditionally expressed in a number of other tissues after exposure to a toxic microenvironment featured by hypoxia and poor nutrient supply. Posttranslational changes including phosphorylation and glycosylation are required for functional activity. Class III β-tubulin’s role in neural development has warranted its use as an early biomarker of neural cell differentiation from multi potent progenitors. TUBB3 inactivation impairs neural progenitor proliferation. Rescue experiments demonstrate the non-interchangeability of TUBB3 with other classes of β-tubulins which cannot restore the phenotype resulting from TUBB3 inactivation. Congenital neurologic syndromes associated with TUBB3 missense mutations demonstrate the critical importance of class III β-tubulin for normal neural development. Overexpression of class III beta tubulin is associated with the resistances of microtubule-targeted cancer drugs in lung cancer cell lines, breast cancer cell lines, and ovarian tumors.

    Antibody type

    Mouse monoclonal Antibody

    Uniprot ID

    SwissProt: Q13509 Human; SwissProt: Q9ERD7 Mouse; SwissProt: Q4QRB4 Rat

    Recombinant

    NO

    Conjugation

    iFluor™ 488

    Host

    Mouse

    Isotype

    IgG2a

    Clone

    A8-D10

    KO/KD

    N/A

    Species reactivity

    Human, Mouse, Rat

    Tested applications

    IF-Cell, FC, IF-Tissue

    Predicted species reactivity

    N/A

    Immunogen

    Synthetic peptide (KLH-coupled) within human/mouse 1-50 aa.

    Storage

    Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

    Form

    Liquid

    Storage buffer

    Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.

    Concentration

    1 mg/mL.

    Purity

    Immunogen affinity purified.

    Signal pathway

    N/A

    Recommended dilutions

    IF-Cell: 1:50; FC: 1:500-1:1, 000; IF-Tissue: 1:200

    Molecular Weight

    Predicted band size: 50 kDa

    Subcellular location

    Cytoplasm. Cytoskeleton. Microtubule.

    Positive control

    HepG2, SH-SY5Y, rat brain tissue, mouse brain tissue.

    Related Products

    Your Dynamic Snippet will be displayed here... This message is displayed because you did not provided both a filter and a template to use.