AffiAB® 488 Conjugated Anti-Actin Antibody [JJ09-29]

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CAT# AFG-HB-4224
Size: 100 μL

386.10 386.1 USD 386.10

386.10

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AffiAB® 488 Conjugated Anti-Actin Antibody [JJ09-29]

All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. α-Actin expression is limited to various types of muscle, whereas β-Actin and γ-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.

Antibody type

Recombinant Rabbit monoclonal Antibody

Uniprot ID

SwissProt: P68133 Human; SwissProt: P68134 Mouse; SwissProt: P68136 Rat

Recombinant

YES

Conjugation

iFluor™ 488

Host

Rabbit

Isotype

IgG

Clone

JJ09-29

KO/KD

N/A

Species reactivity

Human, Mouse, Rat

Tested applications

IF-Cell, IF-Tissue, FC

Predicted species reactivity

N/A

Immunogen

Synthetic peptide within Human Actin aa 45-80 / 377.

Storage

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Form

Liquid

Storage buffer

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS

Concentration

1 mg/mL.

Purity

Protein A affinity purified.

Signal pathway

N/A

Recommended dilutions

IF-Cell: 1:100; IF-Tissue: 1:50; FC: 1:500-1:1, 000

Molecular Weight

Predicted band size: 42 kDa

Subcellular location

Cytoskeleton.

Positive control

NIH/3T3, human colon carcinoma tissue.