AffiAB® 488 Conjugated Alpaca anti-Rabbit IgG FC, Recombinant VHH Monoclonal Antibody (HA1129)

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CAT# AFG-HB-4223
Size: 100 μL

332.10 332.1 USD 332.10

332.10

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    AffiAB® 488 Conjugated Alpaca anti-Rabbit IgG FC, Recombinant VHH Monoclonal Antibody (HA1129)

    Higher resolution imaging due to the Alpaca anti-rabbit IgG Fc, recombinant VHHs’ 10 times smaller size than conventional secondary antibodies. The small size enables better tissue penetration, antigen access and decreases the distance between the epitope and label. Although FITC is still the most popular fluorescent labeling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence. Protein conjugates prepared with iFluor™ 488 dyes are far superior compared to conjugates of fluorescein derivatives such as FITC. iFluor™ 488 conjugates are significantly brighter than fluorescein conjugates and are much more photostable. Additionally, the fluorescence of iFluor™ 488 is not affected by pH (4-10) . This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only at pH above 9. iFluor™ 488 SE dye is reasonably stable and shows good reactivity and selectivity with protein amino groups.

    Antibody type

    Monoclonal secondary

    Uniprot ID

    N/A

    Recombinant

    FALSE

    Conjugation

    iFluor™ 488

    Host

    Alpaca

    Isotype

    IgG

    Clone

    N/A

    KO/KD

    N/A

    Species reactivity

    Rabbit

    Tested applications

    IF-Cell

    Predicted species reactivity

    N/A

    Immunogen

    Fc region of Rabbit IgG.

    Storage

    Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

    Form

    Liquid

    Storage buffer

    Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS

    Concentration

    1ug/ul

    Purity

    Immunogen affinity purified.

    Signal pathway

    N/A

    Recommended dilutions

    IF-Cell: 1:500

    Molecular Weight

    15 kDa

    Subcellular location

    N/A

    Positive control

    N/A

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