AffiAB® HRP Conjugated Anti-Beta tubulin Antibody [1-B11]
Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin. The beta-tubulin (relative molecular weight about 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain. Beta-Tubulin may have bound GTP or GDP. Under certain conditions β-tubulin can hydrolyze its bound GTP to GDP plus Pi, release the Pi, and exchange the GDP for GTP.
Antibody type
Mouse monoclonal Antibody
Uniprot ID
SwissProt: P07437 Human; SwissProt: P99024 Mouse; SwissProt: P69897 Rat
Recombinant
NO
Conjugation
HRP-conjugated
Host
Mouse
Isotype
IgG1
Clone
1-B11
KO/KD
N/A
Species reactivity
Human, Mouse, Rat, Zebrafish
Tested applications
WB, IHC-P
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human Beta tubulin aa 46-95 / 444.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 50% Glycerol.
Concentration
2 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
Parkinson’s disease, Huntington's disease, Amyotrophic lateral sclerosis, Alzheimer disease
Recommended dilutions
WB: 1:1, 000-1:5, 000
; IHC-P: 1:50-1:200
Molecular Weight
50 kDa
Subcellular location
Cytoplasm, Cytoskeleton.
Positive control
NIH-3T3 cell lysate, PC-12 cell lysate, 293 cell lysate, Hela cell lysate, mouse fallopian tube tissue.