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AffiAB® HRP Conjugated Anti-beta Actin Antibody [A2-F6]
Beta actin also know as Cytoplasmic Actin is a 42 kDa, highly conserved protein, ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. β-Actin is a relatively stable cytoskeletal protein normally at a constant level in cells, regardless of experimental treatment or technical procedure. For this reason, measurement of β -Actin is generally used as an internal control for experimental error.
Antibody type
Mouse monoclonal Antibody
Uniprot ID
SwissProt: P60709 Human; SwissProt: P60710 Mouse; SwissProt: P60711 Rat
Recombinant
NO
Conjugation
HRP-conjugated
Host
Mouse
Isotype
IgG1
Clone
A2-F6
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within N-terminal human Beta-actin .
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.2% BSA, 50% Glycerol.
Concentration
2 mg/mL.
Purity
Protein G affinity purified.
Signal pathway
Apotosis, Amyotrophic lateral sclerosis
Recommended dilutions
WB: 1:1, 000-1:5, 000
Molecular Weight
42 kDa
Subcellular location
Cytoplasm.
Positive control
PC12 cell lysate, Hela cell lysate, NIH/3T3 cell lysate.