AffiAB® Anti-Y14 Antibody
RNA-binding protein 8A is a protein that in humans is encoded by the RBM8A gene. This gene encodes a protein with a conserved RNA-binding motif. The protein is found predominantly in the nucleus, although it is also present in the cytoplasm. It is preferentially associated with mRNAs produced by splicing, including both nuclear mRNAs and newly exported cytoplasmic mRNAs. It is thought that the protein remains associated with spliced mRNAs as a tag to indicate where introns had been present, thus coupling pre- and post-mRNA splicing events. Previously, it was thought that two genes encode this protein, RBM8A and RBM8B; it is now thought that the RBM8B locus is a pseudogene. Two alternative start codons result in two forms of the protein, and this gene also uses multiple polyadenylation sites.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: Q9Y5S9 Human; SwissProt: Q9CWZ3 Mouse; SwissProt: Q27W01 Rat
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IHC-P
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human Y14 aa 125-174 / 174.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:2, 000-1:5, 000; IHC-P: 1:200; IF-Cell: 1:50-1:200
Molecular Weight
Predicted band size: 20kDa
Subcellular location
Nucleus, Cytoplasm.
Positive control
Hela, HepG2, Jurkat, HCT116, SW480, MCF-7, RWPE-1, SW620, mouse kidney tissue, mouse heart tissue.