AffiAB® Anti-WDR77 Antibody
Methylosome protein 50 is a protein that in humans is encoded by the WDR77 gene. Non-catalytic component of the methylosome complex, composed of PRMT5, WDR77 and CLNS1A, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The methylosome complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4) , methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: Q9BQA1 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human
Tested applications
WB, IF-Cell, FC
Predicted species reactivity
N/A
Immunogen
Recombinant protein within human WDR77 aa 1-342 / 342.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
PBS (pH7.4) , 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:1, 000; IF-Cell: 1:200; FC: 1:500-1:1, 000
Molecular Weight
Predicted band size: 37 kDa
Subcellular location
Nucleus, Cytoplasm.
Positive control
HepG2 cell lysate, Daudi cell lysate, Hela cell lysate, 293 cell lysate, K562 cell lysate, Hela, HepG2.