AffiAB® Anti-WASL Antibody
Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Involved in mitosis and cytokinesis, via its role in the regulation of actin polymerization. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. Plays a role in dendrite spine morphogenesis.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: O00401 Human; SwissProt: O08816 Rat
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Rat
Tested applications
WB, IF-Cell, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Recombinant protein within Human WASL aa 89-295 / 505.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:2, 000; IF-Cell: 1:50-1:200; IHC-P: 1:50; FC: 1:50-1:100
Molecular Weight
Predicted band size: 55 kDa
Subcellular location
Cytoplasm. Cytoskeleton. Nucleus.
Positive control
SH-SY-5Y, SiHa, SK-Br-3, rat brain tissue, human small intestine tissue.