AffiAB® Anti-VPS28 Antibody [JE55-08]

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CAT# AFG-HB-4111
Size: 100 μL

496.50 496.5 USD 496.50

496.50

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AffiAB® Anti-VPS28 Antibody [JE55-08]

Vacuolar protein sorting-associated protein 28 homolog is a protein that in humans is encoded by the VPS28 gene. This gene encodes a protein involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. The encoded protein is one of the three subunits of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated protein. The two other subunits of ESCRT-I are vacuolar protein sorting 23 (VPS23) , also known as tumor susceptibility gene 101 (TSG101) , and vacuolar protein sorting 37 (VPS37) . Two alternative transcripts encoding different isoforms have been described. Additional alternative transcripts may exist but the proteins encoded by these transcripts have not been verified experimentally. VPS28 has been shown to interact with TSG101.

Antibody type

Recombinant Rabbit monoclonal Antibody

Uniprot ID

SwissProt: Q9UK41 Human; SwissProt: Q9D1C8 Mouse; SwissProt: B5DEN9 Rat

Recombinant

YES

Conjugation

Non-conjugated

Host

Rabbit

Isotype

IgG

Clone

JE55-08

KO/KD

N/A

Species reactivity

Human, Mouse, Rat

Tested applications

WB, IF-Cell, IHC-P, FC

Predicted species reactivity

N/A

Immunogen

Recombinant protein within Human VPS28 aa 100-221 / 221.

Storage

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Form

Liquid

Storage buffer

1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 mg/mL.

Purity

Protein A affinity purified.

Signal pathway

N/A

Recommended dilutions

WB:1:500-1:1, 000; IF-Cell:1:50-1:200; IHC-P:1:50-1:200; FC:1:50-1:100

Molecular Weight

25 kDa

Subcellular location

Late endosome membrane, Cell membrane.

Positive control

Mouse testis tissue lysate, human small intestine tissue lysate, rat bone marrow tissue lysate, N2A, human colon tissue, human prostate carcinoma tissue, human uterus tissue, mouse testis tissue, SH-SY5Y.