AffiAB® Anti-Vitronectin Antibody [ST49-02]
Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway. The protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P04004 Human; SwissProt: P29788 Mouse; SwissProt: Q3KR94 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
ST49-02
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IF-Tissue, IHC-P
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human Vitronectin aa 429-478 / 478.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
Cardiovascular, PI3K-AKT
Recommended dilutions
WB: 1:1, 000-1:5, 000; IF-Cell: 1:50-1:200; IF-Tissue: 1:50-1:200; IHC-P: 1:50-1:400
Molecular Weight
Predicted band size: 54 kDa
Subcellular location
Secreted.
Positive control
Human liver tissue lysate, human serum lysate, A431, A549, HepG2, MCF-7, human liver tissue, mouse liver tissue, mouse skin tissue, human skin tissue, human skin tissue, rat skin tissue.