AffiAB® Anti-Vimentin Antibody
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Research studies have shown that vimentin is present in sarcomas, but not carcinomas, and its expression is examined in conjunction with that of other markers to distinguish between the two. Vimentin's dynamic structural changes and spatial re-organization in response to extracellular stimuli help to coordinate various signaling pathways.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P08670 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
Knockout validated
Species reactivity
Human
Tested applications
WB, IF-Cell, IHC-P
Predicted species reactivity
Mouse, Rat
Immunogen
Synthetic peptide within residues of Human Vimentin aa 417-466 / 466.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
Cancer, Neuroscience
Recommended dilutions
WB: 1:1, 000-1:2, 000; IF-Cell: 1:100-1:200; IHC-P: 1:200
Molecular Weight
Predicted band size: 54 kDa
Subcellular location
Cytoplasm, Cell membrane, Cytoskeleton, Intermediate filament, Membrane, Nucleus.
Positive control
Hela cell lysate, A549 cell lysate, 293T cell lysate, Hela, human breast carcinoma tissue, Wild-type Hela whole cell lysate.