AffiAB® Anti-VEGF Antibody
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P15692 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
Knockdown validated
Species reactivity
Human
Tested applications
WB, IF-Cell, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within N-terminal human VEGFA.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
VEGF signaling pathway, HIF-1 signaling pathway, PI3K-AKT, Cancer, Cardiovascular, Rheumatoid arthritis
Recommended dilutions
WB: 1:500-1:1, 000; IF-Cell: 1:200; IHC-P: 1:200; FC: 1:100-1:200
Molecular Weight
27 kDa
Subcellular location
Secreted.
Positive control
MCF-7, Hela, Jurkat, HUVEC, human brain tissue, human lung carcinoma tissue, HepG2.