![AffiAB® Anti-VCP Antibody [JM11-15]](/web/image/product.product/89280/image_1024/%5BAFG-HB-4066%5D%20AffiAB%C2%AE%20Anti-VCP%20Antibody%20%5BJM11-15%5D%20?unique=a48b62f)
AffiAB® Anti-VCP Antibody [JM11-15]
Valosin containing protein (VCP) , also designated TERA (for transitional endoplasmic reticulum ATPase) or p97, is a member of the AAA family of ATPases, which are involved in a variety of cellular activities. VCP is the mammalian homolog of Saccharomyces cerevisiae Cdc48, a protein essential for the completion of mitiosis in yeast. VCP is thought to be involved in a variety of membrane functions and in the regulation of the cell cycle. It associates with ubiquitinated IκB-α as well as with the 26S Proteosome, indicating a potential role for VCP in the proteosome-mediated degradation of IκB-α.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P55072 Human; SwissProt: Q01853 Mouse; SwissProt: P46462 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JM11-15
KO/KD
N/A
Species reactivity
Human, Mouse, Rat, Zebrafish
Tested applications
WB, IF-Cell, IF-Tissue, IHC-P, FC, IP
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human VCP aa 1-48 / 806.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
Neuroscience, Amyotrophic lateral sclerosis
Recommended dilutions
WB: 1:1, 000-1:5, 000; IF-Cell: 1:50-1:200; IF-Tissue: 1:50-1:200; IHC-P: 1:50-1:200; FC: 1:50-1:100; IP: Use at an assay dependent concentration.
Molecular Weight
Predicted band size: 89 kDa
Subcellular location
Cytoplasm, Endoplasmic reticulum, Nucleus.
Positive control
SH-SY5Y cell lysate, HepG2 cell lysate, Hela cell lysate, hybrid fish (crucian-carp) heart tissue lysates, A549, Hela, SW480, human colon carcinoma tissue, human breast carcinoma tissue, human liver tissue, mouse colon tissue, rat brain tissue, zebrafish tissue, HL-60.