AffiAB® Anti-VASP Antibody [SY02-16]
The Wiskott-Aldrich syndrome (WAS) is characterized by thrombocytopenia, eczema, defects in cell-mediated and humoral immunity, and a propensity for lymphoproliferative diseases. The syndrome is the result of a mutation in the gene encoding a proline-rich protein termed WASP. WASP has been identified as a downstream effector of Cdc42 and has been implicated in Actin polymer-ization and cytoskeletal organization. A distantly related protein, VASP (vaso-dilator-stimulated phosphoprotein) , is involved in the maintenance of cytoarchitecture by interacting with Actin-like filaments. VASP shares a limited degree of homology with the amino-terminus of WASP, which is frequently mutated in WAS patients. An established substrate of cAMP and cGMP de-pendent kinases, VASP is phosphorylated on a regulatory Serine residue 157 and localizes to focal adhesions, microfilaments and highly active regions of the plasma membrane. VASP is highly expressed in human platelets and, like WASP, may play a role in cytoskeletal organization.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P50552 Human
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
SY02-16
KO/KD
N/A
Species reactivity
Human
Tested applications
WB, IP
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human VASP aa 51-100 / 380.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
cGMP-PKG signaling pathway
Recommended dilutions
WB: 1:1, 000-1:2, 000
Molecular Weight
46 kDa
Subcellular location
Cytoplasm, Cell junction, Cell projection.
Positive control
HT29, MCF-7, Hela.