AffiAB® Anti-USP13 Antibody
The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP13 (ubiquitin specific peptidase 13) , also known as ISOT3 (Isopeptidase T-3) , is an 863 amino acid protein that belongs to the peptidase C19 family and contains one UBP-type zinc finger and two UBA domains. Highly expressed in testicular and ovarian tissue, USP13 functions to catalyze the water-dependent conversion of a ubiquitin C-terminal thioester to a thiol and a free ubiquitin.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: Q92995 Human; SwissProt: Q5BKP2 Mouse
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, FC, IHC-P
Predicted species reactivity
N/A
Immunogen
Recombinant protein within Human USP13 aa 570-776 / 863.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:2, 000; IF-Cell: 1:50-1:200; FC: 1:50-1:100; IHC-P: 1:100-1:200
Molecular Weight
97 kDa
Subcellular location
Nucleus. Cytosol.
Positive control
293T, Daudi, mouse testis tissue, rat brain tissue, A431, MG-63.