AffiAB® Anti-USP10 Antibody [JU32-62]
The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP10 (ubiquitin specific peptidase 10) , also known as UBPO, is a 798 amino acid protein that belongs to the ubiquitin-specific protease family of cysteine proteases. Expressed in a variety of tissues, USP10 functions to catalyze the cleavage of ubiquitin from ubiquitin-conjugated protein substrates, possibly playing a role in the activity of the DNA-bound androgen receptor complex.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: Q14694 Human
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JU32-62
KO/KD
N/A
Species reactivity
Human
Tested applications
WB, IF-Cell, IF-Tissue, IHC-P, IP
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human USP10 aa 28-77 / 798.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:2, 000; IF-Cell: 1:50-1:200; IF-Tissue: 1:50-1:200; IHC-P: 1:50-1:200
Molecular Weight
110 kDa
Subcellular location
Cytoplasm. Nucleus.
Positive control
SiHa cell lysate, A549 cell lysate, Hela, LOVO, MCF-7, human liver tissue tissue, human thyroid grand tissue tissue, human colon carcinoma tissue tissue, human spleen tissue, human placenta tissue.