AffiAB® Anti-UBE2D3 Antibody
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P61077 Human; SwissProt: P61079 Mouse; SwissProt: P61078 Rat
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IHC-P
Predicted species reactivity
N/A
Immunogen
Recombinant full length protein of human UBE2D3.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:1, 000; IF-Cell: 1:50-1:100; IHC-P: 1:100
Molecular Weight
Predicted band size: 17 kDa.
Subcellular location
Cell membrane, Endosome membrane.
Positive control
Jurkat cell lysate, 293T cell lysate, MCF-7 cell lysate, rat liver tissue lysate, mouse lung tissue lysate, SW620, mouse brain tissue.