![AffiAB® Anti-Ube2B Antibody [JE56-12]](/web/image/product.product/89209/image_1024/%5BAFG-HB-3994%5D%20AffiAB%C2%AE%20Anti-Ube2B%20Antibody%20%5BJE56-12%5D%20?unique=a48b62f)
AffiAB® Anti-Ube2B Antibody [JE56-12]
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P63146 Human
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JE56-12
KO/KD
N/A
Species reactivity
Human, Zebrafish
Tested applications
WB, IHC-P
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within N-terminal Human Ube2B.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:2, 000
; IHC-P: 1:50-1:200
Molecular Weight
17 kDa
Subcellular location
Nucleus, Cell membrane.
Positive control
A431 cell lysate, THP-1 cell lysate, HepG2 cell lysate, zebrafish tissue lysates, human breast carcinoma tissue, human fetal skeletal muscle tissue.