AffiAB® Anti-TTL Antibody
TTL is a cytosolic enzyme involved in the posttranslational modification of alpha-tubulin (see MIM 602529) . Alpha-tubulin within assembled microtubules is detyrosinated over time at the C terminus. After microtubule disassembly, TTL restores the tyrosine residues and consequently participates in a cycle of tubulin detyrosination and tyrosination (Erck et al., 2003) .
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: Q8NG68 Human; SwissProt: P38585 Mouse; SwissProt: Q9QXJ0 Rat
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within human TTL aa 230-270.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:1, 000
; IHC-P: 1:50-1:200
; FC: 1:50-1:100
Molecular Weight
43 kDa
Subcellular location
Nucleoplasm, Vesicles.
Positive control
Human heart tissue lysates, rat brain tissue, mouse brain tissue, SH-SY5Y.