AffiAB® Anti-TTK Antibody
This gene encodes a dual specificity protein kinase with the ability to phosphorylate tyrosine, serine and threonine. Associated with cell proliferation, this protein is essential for chromosome alignment at the centromere during mitosis and is required for centrosome duplication. It has been found to be a critical mitotic checkpoint protein for accurate segregation of chromosomes during mitosis. Tumorigenesis may occur when this protein fails to degrade and produces excess centrosomes resulting in aberrant mitotic spindles. Alternative splicing results in multiple transcript variants.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P33981 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human
Tested applications
WB, IHC-P, IF-Cell, FC
Predicted species reactivity
N/A
Immunogen
Recombinant protein within human TTK aa 1-300.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 1% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:1, 000-1:2, 000; IHC-P: 1:100; IF-Cell: 1:50; FC: 1:500-1:1, 000
Molecular Weight
Predicted band size: 97 kDa.
Subcellular location
Spindle, nucleus, cytoplasm, kinetochore, membrane.
Positive control
Human placenta tissue lysates, human lung tissue, human thyroid tissue, human colon carcinoma tissue, human skin tissue, human breast carcinoma tissue, human esophagus tissue, human placenta tissue, SH-SY5Y, MG-63.