AffiAB® Anti-TRIM28 Antibody
TRIM28, also known as transcriptional intermediary factor 1β (TIF1β) and KAP1, is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. TIF1-beta mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: Q13263 Human; SwissProt: Q62318 Mouse
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IF-Cell, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within human TRIM28 aa 415-459.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
Cancer
Recommended dilutions
WB: 1:500-1:2, 000; IF-Cell: 1:50-1:200; IHC-P: 1:50-1:200; FC: 1:50-1:100
Molecular Weight
110 kDa
Subcellular location
Nucleus
Positive control
Hela, NCCIT, F9, PC-3M, MCF-7, A431, human liver cancer tissue, human spleen tissue, human breast cancer tissue, human kidney tissue, mouse colon tissue, mouse kidney tissue.