AffiAB® Anti-TRIM25 Antibody
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: Q14258 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Rat
Tested applications
WB, IHC-P
Predicted species reactivity
N/A
Immunogen
Recombinant protein within human TRIM25 aa 20-320/630.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
NF-KB signaling pathway
Recommended dilutions
WB: 1:500-1:2, 000
; IHC-P: 1:100-1:500
Molecular Weight
Predicted band size: 71 kDa.
Subcellular location
Cytoplasm, Stress granule.
Positive control
Jurkat cell lysate, 293 cell lysate, SK-Br-3 cell lysates, rat bladder tissue.