AffiAB® Anti-TRAF6 Antibody
TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. This protein mediates the signaling not only from the members of the TNF receptor superfamily, but also from the members of the Toll/IL-1 family. Signals from receptors such as CD40, TNFSF11/TRANCE/RANKL and IL-1 have been shown to be mediated by this protein. This protein also interacts with various protein kinases including IRAK1/IRAK, SRC and PKCzeta, which provides a link between distinct signaling pathways. This protein functions as a signal transducer in the NF-kappaB pathway that activates IkappaB kinase (IKK) in response to proinflammatory cytokines.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: Q9Y4K3 Human; SwissProt: P70196 Mouse; SwissProt: B5DF45 Rat
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
IF-Cell, IHC-P, WB
Predicted species reactivity
Chicken
Immunogen
Synthetic peptide within C-terminal human TRAF6.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
Cancer, NF-KB signaling pathway, Autophagy Immunology & Inflammation, MAPK signaling pathway
Recommended dilutions
WB: 1:500-1:1, 000; IF-Cell: 1:100-1:200; WB: 1:500-1:2, 000
Molecular Weight
Predicted band size: 60 kDa
Subcellular location
Cytoplasm, nucleus
Positive control
Hela, MCF-7, F9, PC12, hybrid fish (crucian-carp) heart tissue lysate.