AffiAB® Anti-Topoisomerase II alpha Antibody [A3F12]
DNA topoisomerase IIα is a human enzyme encoded by the TOP2A gene. Topoisomerase IIα relives topological DNA stress during transcription, condenses chromosomes, and separates chromatids. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several chemotherapy agents and a variety of mutations in this gene have been associated with the development of drug resistance. [citation needed] Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. TOP2A has been shown to interact with SMURF2, HDAC1, CDC5L, Small ubiquitin-related modifier 1 and P53.
Antibody type
Mouse monoclonal Antibody
Uniprot ID
SwissProt: P11388 Human; SwissProt: Q01320 Mouse; SwissProt: P41516 Rat
Recombinant
NO
Conjugation
Non-conjugated
Host
Mouse
Isotype
IgG1
Clone
A3F12
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IHC-P
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within human Topoisomerase II alpha aa1480-1531.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 mg/mL.
Purity
Protein G affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:2, 000
; IHC-P: 1:200
Molecular Weight
174/178/182 kDa
Subcellular location
Cytoplasm, Nucleus.
Positive control
A549 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, human breast carcinoma tissue.