AffiAB® Anti-Themis Antibody [JE55-73]
This gene encodes a protein that plays a regulatory role in both positive and negative T-cell selection during late thymocyte development. The protein functions through T-cell antigen receptor signaling, and is necessary for proper lineage commitment and maturation of T-cells. Alternative splicing results in multiple transcript variants.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: Q8N1K5 Human
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JE55-73
KO/KD
N/A
Species reactivity
Human, Rat
Tested applications
WB, IF-Cell, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within C-terminal Human Themis.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:2, 000; IF-Cell: 1:50-1:100; IHC-P: 1:50-1:200; FC: 1:50-1:100
Molecular Weight
73 kDa
Subcellular location
Nucleus, Cytoplasm.
Positive control
Jurkat cell lysates, 293T, rat spleen tissue, human appendix tissue, human spleen tissue, Jurkat.