AffiAB® Anti-TCF7L2/TCF4 Antibody
TCF7L2 is a transcription factor influencing the transcription of several genes thereby exerting a large variety of functions within the cell. It is a member of the Wnt signaling pathway. Stimulation of the pathway leads to the association of β-catenin with BCL9, translocation to the nucleus, and association with TCF7L2, which in turn results in the activation of Wnt target genes, specifically repressing proglucagon synthesis in enteroendocrine cells. TCF7L2 is implicated in a large variety of diseases. Several single nucleotide polymorphisms are associated with type 2 diabetes. In European populations it was found to be a major determinant of type 2 risk. A frameshift mutation of TCF7L2 is implicated in colorectal cancer .Variants of the gene are most likely involved in many other cancer types
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: Q9NQB0 Human; SwissProt: Q924A0 Mouse; SwissProt: D3Z9D1 Rat
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IHC-P, IF-Cell, FC
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within N-terminalHuman TCF4 aa 1-50 / 619.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:1, 000-1:2, 000; IF-Cell: 1:500-1:2, 000; IHC-P: 1:100-1:500; FC: 1:50-1:100
Molecular Weight
Predicted band size: 68 kDa
Subcellular location
Nucleus
Positive control
Hela, HepG2, HCT116, SW480, rat kidney tissue, human tonsil tissue, human colon cancer tissue, human breast cancer tissue.