AffiAB® Anti-TCEA1 Antibody [JE64-90]
Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P23193 Human; SwissProt: P10711 Mouse; SwissProt: Q4KLL0 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JE64-90
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IHC-P
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within human TCEA1 aa 201-250/301.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:2, 000
; IHC-P: 1:400-1:2, 000
Molecular Weight
Predicted band size: 34 kDa
Subcellular location
Nucleus.
Positive control
K562 cell lysate, HUVEC cell lysate, Hela cell lysate, Jurkat cell lysate, mouse large intestine tissue, mouse pancreas tissue lysates, rat kidney tissue, human lung carcinoma tissue.