AffiAB® Anti-Tat-SF1 Antibody [JE54-89]
The protein encoded by this gene functions as a cofactor for the stimulation of transcriptional elongation by HIV-1 Tat, which binds to the HIV-1 promoter through Tat-TAR interaction. This protein may also serve as a dual-function factor to couple transcription and splicing and to facilitate their reciprocal activation. Alternatively spliced transcript variants have been found for this gene.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: O43719 Human; SwissProt: Q8BGC0 Mouse
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JE54-89
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IF-Cell, IHC-P
Predicted species reactivity
N/A
Immunogen
Recombinant protein within Human Tat-SF1 aa 1-140 / 755.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB:1:500-1:1, 000; IF-Cell:1:50-1:100; IHC-P:1:50-1:200
Molecular Weight
Predicted band size 86 kDa.
Subcellular location
Nucleus.
Positive control
CRC cell lysate, MCF-7 cell lysate, MCF-7, RWPE-1, human breast tissue, human stomach carcinoma tissue, mouse liver tissue, mouse colon tissue, mouse kidney tissue.