![AffiAB® Anti-T7 tag Antibody [4-C2]](/web/image/product.product/88988/image_1024/%5BAFG-HB-3759%5D%20AffiAB%C2%AE%20Anti-T7%20tag%20Antibody%20%5B4-C2%5D%20?unique=8544d55)
AffiAB® Anti-T7 tag Antibody [4-C2]
The T7 tag is an epitope tag composed of an 11-residue peptide encoded from the leader sequence of the T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag has been used extensively as a general epitope tag in many expression vectors including the pET system that is based on T7 RNA polymerase expression systems. This antibody recognizes the T7-tag fused to either the amino- or carboxy-terminus of targeted proteins.
Antibody type
Mouse monoclonal Antibody
Uniprot ID
N/A
Recombinant
NO
Conjugation
Non-conjugated
Host
Mouse
Isotype
IgG1
Clone
4-C2
KO/KD
N/A
Species reactivity
N/A
Tested applications
WB, ELISA, IP, IF-Cell
Predicted species reactivity
N/A
Immunogen
Synthetic peptide CMASMTGGQQMG.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:1, 000; ELISA: Use at an assay dependent concentration.; IF-Cell: Use at an assay dependent concentration.; IP: Use at an assay dependent concentration.
Molecular Weight
N/A
Subcellular location
N/A
Positive control
T7-tagged recombinant protein.